Journal: Molecular Therapy. Methods & Clinical Development

Article Title: Quantification of full and empty particles of adeno-associated virus vectors via a novel dual fluorescence-linked immunosorbent assay

doi: 10.1016/j.omtm.2024.101291

Figure Lengend Snippet: Application of dFLISA for quantification of different AAV serotypes (A) Capsid titer quantification. (B) Quantification of genome titer. (C) Detection of FP ratio. dFLISA experiments were performed in duplicate for AAV2 and AAV8 vectors. Expected values derived from BS-AUC (dark navy blue) were compared with actual values for three parameters, specifically capsid titer (red), genomic titer (green), and FP ratio (light green). Results are the average of duplicated wells.

Article Snippet: Lyophilized mouse anti-AAV2 monoclonal antibody (A20) and lyophilized mouse anti-AAV8 monoclonal antibody (ADK8) were purchased from Progen, (Heidelberg, Germany).

Techniques: Derivative Assay

Journal: eLife

Article Title: Paradoxical imbalance between activated lymphocyte protein synthesis capacity and rapid division rate

doi: 10.7554/eLife.89015

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-mouse mouse CD45.1 Monoclonal Antibody (A20), APC, eBioscience , Invitrogen , Cat#: 17-0453-82 RRID: AB_469398 , FACS (0.75 μl per test).

Techniques: Transgenic Assay, Western Blot, Recombinant, Activation Assay, Virus, DC Protein Assay

Journal: eLife

Article Title: Complex subsets but redundant clonality after B cells egress from spontaneous germinal centers

doi: 10.7554/eLife.81012

Figure Lengend Snippet:

Article Snippet: Antibody , anti-CD45.1-APC (A20)(mouse monoclonal) , Biolegend , 110714 , 1:300.

Techniques: Recombinant, Cell Isolation, Software

Journal: Cancers

Article Title: A20 as a Potential New Tool in Predicting Recurrence and Patient's Survival in Oral Squamous Cell Carcinoma.

doi: 10.3390/cancers15030675

Figure Lengend Snippet: Figure 1. Histomorphology (H&E; (A,D,G)) and CD3 and A20 IHC stainings at central and peripheral tumor areas and the associated non-neoplastic mucosa of a representative keratinizing OSCC case of the TMA cohort. Whereas only few or no stromal CD3+ T cells within the tumor center (B,C) and non-neoplastic mucosa (H,I), respectively, show A20 IHC expression, high A20 expression was observed within the stromal CD3+ T cells at the tumor periphery (E,F).

Article Snippet: For A20 IHC, the staining protocol was manually performed using a mouse monoclonal anti-A20 antibody (dilution 1:50 clone A20(A-12) sc-166692; Santa Cruz Biotechnology Inc., Dallas, TX, USA) with an incubation time of 30 min at room temperature (RT), followed by DAB and counterstaining with hematoxylin.

Techniques: Expressing

Journal: Cancers

Article Title: A20 as a Potential New Tool in Predicting Recurrence and Patient's Survival in Oral Squamous Cell Carcinoma.

doi: 10.3390/cancers15030675

Figure Lengend Snippet: Figure 2. (A) Analysis of relative A20 IHC expression in central and peripheral OSCC as well as non-malignant mucosa (n = 172); (B) Expression of stromal CD3+ T cells in the tumor center, the tumor periphery and non-malignant mucosa; (C) A20 IHC expression within stromal CD3+ T cells in tumor center, tumor periphery as well as non-malignant mucosa; **** p < 0.0001, n.s. = not significant.

Article Snippet: For A20 IHC, the staining protocol was manually performed using a mouse monoclonal anti-A20 antibody (dilution 1:50 clone A20(A-12) sc-166692; Santa Cruz Biotechnology Inc., Dallas, TX, USA) with an incubation time of 30 min at room temperature (RT), followed by DAB and counterstaining with hematoxylin.

Techniques: Expressing